Auxotrophic babies

There's always perspectives. And it's up to an individual to choose which side of the coin he/she chooses to side on. Working in pairs in any given biology experiment can be advantages, considering one partner can assume the role of a supervisor, and perform immediate quality checks on his partner, just in case if any mistakes are made. And if there are, it can be immediately identified and the appropriate corrective measures be taken asap.

Each partner may take turns to carry out certain procedures, but there comes some risk that if one partner performed some key step wrongly, his partner would unfortunately suffer the fatal consequnences too.

Today's lab was challenging, quite a number of tasks, including DNA extraction, agar platings, blah blah. I know Lie Cong mentioned that i've said this too many times but i'll nag about this point again, NS can really cause one's brain to rust! Haha.

At least 2 other course mates whom walked past me asked me the following;

"You're doing today's experiment by yourself??"

They were rather surprised by my answer and one did offered help if I needed any. Thank God for samaritans. I really appreciate it :)




Looking on the positive side tonight, Victor wasn't around today due to work commitments i guess, and I take this as a perfect opportunity to work alone and improve my techniques. However there were moments when i wished I had someone beside helping, even simple tasks like labeling the correct microcentrifuge tubes and/or petri dishes. And i'm really hoping that I didnt make any mistakes man. Not that i know currently. haha.

And i'm praying for successful transformation of my Bacillus subtilis. Please grow in well the media without tryptophan! I've given you ample time to take up nucleic acid containing the Trp gene! don't let me down my little babies!



As expected, i was the last who finished everything in the lab. But oh well, does it matter? :)


Time for an early night!